山東大學(xué)生命科學(xué)學(xué)院導(dǎo)師：倪金鳳 正文

　　倪金鳳博士，教授
　　
　　通訊地址:山東大學(xué)微生物技術(shù)國家重點(diǎn)實(shí)驗(yàn)室微生物樓518室
　　山東省濟(jì)南市山大南路27號，郵編250100
　　Tel：0531-88363323，Tel/Fax 0531-88362928
　　jinfgni＠sdu.edu.cn
　　
　　教育背景及工作經(jīng)歷:
　　蘭州大學(xué)生物系細(xì)胞生物學(xué)理科碩士，日本岐阜大學(xué)生物資源科學(xué)應(yīng)用微生物學(xué)博士。日本農(nóng)業(yè)生物資源研究所研究助理，生研機(jī)構(gòu)特別研究員，日本學(xué)術(shù)振興會外國人特別研究員（JSPS）。2004年4月回國任山東大學(xué)微生物技術(shù)國家重點(diǎn)實(shí)驗(yàn)室副教授、碩士生導(dǎo)師。2009年12月被聘為重點(diǎn)實(shí)驗(yàn)室教授。
　　
　　研究方向:
　　特殊環(huán)境（極端環(huán)境及白蟻腸道）中微生物及微生物酶的研究與應(yīng)用。
　　關(guān)鍵詞：極端酶，白蟻纖維素酶，超嗜熱古菌，遺傳轉(zhuǎn)化
　　具體包括以下幾方面：
　　1. 白蟻木質(zhì)纖維素降解酶系研究。通過分子生物學(xué)和組學(xué)方法研究白蟻降解木質(zhì)纖維素機(jī)理；
　　2. 極端環(huán)境酶研究。構(gòu)建文庫，篩選基因，進(jìn)行高效表達(dá)，為產(chǎn)業(yè)化應(yīng)用奠定基礎(chǔ)；
　　3. 古菌遺傳轉(zhuǎn)化系統(tǒng)研究。構(gòu)建敲除表達(dá)載體，研究參與古菌DNA代謝重要蛋白的生理功能。
　　
　　主要科研項(xiàng)目：
　　1. 國家重點(diǎn)基礎(chǔ)研究發(fā)展計劃（973）“木質(zhì)纖維素資源高效生物降解轉(zhuǎn)化中的關(guān)鍵科學(xué)問題研究”子課題：木質(zhì)纖維素生物降解多樣性、新降解因子及相關(guān)機(jī)理。
　　2. 國家自然科學(xué)基金重點(diǎn)項(xiàng)目: 超嗜熱古菌DNA重組修復(fù)解旋酶和核酸酶的作用機(jī)制及體內(nèi)功能。
　　3. 國家自然科學(xué)基金面上項(xiàng)目：白蟻后腸共生微生物由來新木質(zhì)纖維降解酶基因的篩選與鑒定。
　　4. 國家自然科學(xué)基金面上項(xiàng)目：利用硫化葉菌異源表達(dá)超嗜熱古菌重組蛋白的研究。
　　
　　招收研究生情況
　　招生專業(yè): 微生物學(xué)，生物化學(xué)與分子生物學(xué)
　　每年招收碩士、博士研究生數(shù)名, 歡迎有志于以上科學(xué)研究的學(xué)子們踴躍報考。另外招收博士后若干名。
　　
　　發(fā)表論文：
　　Hong Y, Chu M, Li Y, Ni J, Sheng D, Hou G, She Q, Shen Y*. Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair (2011), doi:10.1016/j.dnarep.2011. 10.009。
　　Zheng T, Huang H, Zhang C, Ni J, She Q, Shen Y*. (2011) Development of simvastatin selection marker for hyperthermophilic acidophile, Appl. Environ. Microbiol., doi:10.1128/AEM.06095 -11
　　Wei T, Zhang S, Hou L, Ni J, Sheng D, Shen Y*.(2011) The carboxyl terminal of the archaeal nuclease NurA is involved in the interaction with single-stranded DNA-binding protein and dimer formation. Extremophiles. 15：11-18.
　　Ma X, Hong Y, Han W, Sheng D, Ni J, Hou G, Shen Y*. (2010) Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting. Extremophiles. 14：1-10.
　　Wu Y, Chi SL and Ni J*. Isolation of a strain of Variovorax sp. R3063 with cellulase activity from termite. (2011) Chinese Journal of Applied Entomology. 48: 33-28
　　Ni J.*, Takehara M and Watanabe H. (2010) Identification of Activity Related Amino Acid Mutations of a GH9 Termite Cellulase. Bioresource Technology. 101:6438-6443.
　　Wu L., Liu, B., Hong, Y.,Sheng, D., Shen, Y., Ni, J.*(2010) Residue Tyr224 is critical for the thermostability of Geobacillus sp. RD-2 lipase, Biotechnol Lett. 32:107-112.
　　Liu, B., Wu L., Liu, T., Hong, Y.,Shen, Y., Ni, J.*(2009)A MOFRL family glycerate kinase from the thermophilic crenarchaeon Sulfolobus tokodaii with unique enzymatic properties, Biotechnol Lett. 31:1937-1941.
　　Wang F, Zhang, S. Huang, Q,Shen, Y andNi, J.* (2009) Advance in genetic manipulation systems of Hyperthermophilic archaea Acta Microbiologica Sinica 49, 1418-1423.
　　Huang Q, Shen Y and Ni, J.* Optimization of selective conditions for the selection of uracil auxotrophs of thermophilic archaea Sulfolobus tokodaii. (2008) Journal of Shandong University (Natural Science), 43, 6-10.
　　Lu S, Li Z, Wang Z, Ma X, Sheng D, Ni J, Shen Y. 2008）Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii. Biochem Biophys Res Commun. 376:369-74.
　　Li Z., Lu S., Hou. G, Ma X., Sheng D., Ni J., and Shen Y. (2008) Hjm/hel308a DNA helicase from Sulfolobus tokodaii promotes replication fork regression and interacts with hjc endonuclease in vitro. J. Bacteriol.190:3006-17.
　　Zhang S, Wei W., Hou. G, Zhang C, Liang P., Ni J., Sheng D., Shen Y. (2008) Archaeal DNA helicase HerA interacts with Mre11 homologue and unwinds blunt-ended double-stranded DNA and recombination intermediates. DNA Repair 7:380–391.
　　Wei T, Zhang S, Zhu S, Sheng D, Ni J, Shen Y. (2008) Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5’-3’ nuclease NurA. Biochem Biophys Res Commun. 367:523-9.
　　Sheng D, Li M, Jiao J, Ni J, Shen Y.（2008）Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaii.Sci China C Life Sci. 51:60-5.
　　Sheng D, Zhu S, Wei T, Ni J, Shen Y. (2008) The in vitro activity of a Rad55 homologue from Sulfolobus tokodaii, a candidate mediator in RadA-catalyzed homologous recombination. Extremophiles 12:147-157.
　　Hong Y, Wu L, Liu B, Peng C, Sheng D, Ni J, Shen Y. (2008) Characterization of a glucan phosphorylase from the thermophilic archaeon Sulfolobus tokodaii strain 7. J. Mol. Catal. B: Enzy. 54:27-34.
　　Sheng D., Zhu S., Li M., Jiao, Ni J., and Shen Y. (2008) Cloning, expression and radiation inducibility of RadA from the hyperthermophilic archaeon Sulfolobus tokodaii. Acta Microbiologica Sinica 48:1-6.
　　Ni J., Takehara M, Miyazawa M and Watanabe H. (2007) Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability. Protein Eng. Des. Sel. 20, 535-542.
　　Ni J.,Tokuda G, Takehara M and Watanabe H. (2007) Heterologous expression and enzymatic characterization of β-glucosidase from the drywood-eating termite, Neotermes koshunensis. Appl. Entomol. Zool. 42, 457-463.
　　Liu, B. Hong, Y., Wu L., Li, Z.,Ni J., Sheng, D., Shen, Y. (2007) A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic archaeon Pyrococcus horikoshii: gene cloning, expression and characterization. Extremophiles 11:733-739.
　　Liu B, Li Z, Hong Y,Ni J., Sheng D, Shen Y. (2006) Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol Lett. 28:1655-1660.
　　Liu, B., Ni J., Shen Y. (2006) Cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeon Pyrococcus horikoshii. Acta Microbiologica Sinica 46:255-258.
　　Ni J., Takehara, M and Watanabe H. (2005) Heterologous overexpression of a mutant termite cellulase gene in E.coli by DNA shuffling of four orthologous parental cDNAs, Biosci. Biotechnol. Biochem. 69: 1711-1720.
　　Ni J., Sasaki, Y., Tokuyama, S., Sogabe, A., and Tahara, Y. (2002) Conversion of a typical catalase from Bacillus sp. TE124 to a catalase-peroxidase by directed evolution. J. Biosci. Bioeng. 93, 31-36.
　　Ni J., Tokuyama, S., Sogabe, A., Kawamura, Y., and Tahara, Y. (2001) Cloning and high expression of catalase gene from Bacillus sp. TE124. J. Biosci. Bioeng. 91, 422-424.

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