山東大學(xué)生命科學(xué)學(xué)院導(dǎo)師：申玉龍 正文

　　申玉龍Yulong Shen，博士，教授, 博士生導(dǎo)師
　　
　　通訊地址: 山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室微生物樓526室
　　山東省濟(jì)南市山大南路27號(hào)，郵編250100
　　Tel：0531-88362928，Tel/Fax 0531-88362928
　　yulgshen＠sdu.edu.cn
　　
　　教育背景:
　　1982至1989年于蘭州大學(xué)生物系學(xué)習(xí)并獲得碩士學(xué)位。1993至1996年就讀于英國(guó)伯明翰大學(xué)生物系，獲得細(xì)胞與分子生物學(xué)方向理學(xué)博士學(xué)位。1997-1999被聘為日本學(xué)術(shù)學(xué)術(shù)振興會(huì)博士后(STA fellowship)。1999至2003, 作為研究員任職于日本產(chǎn)業(yè)技術(shù)綜合研究所。2003年11月至今, 受聘于山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室。多年來(lái)一直從事古菌生物化學(xué)和分子生物學(xué)的研究工作，主要致力于古菌DNA重組修復(fù)與基因組穩(wěn)定性維持機(jī)制以及極端環(huán)境微生物資源研究。在J. Biol. Chem., Nucleic Acids Res., J. Bacteriol. , DNA Repair和Appl. Environ. Microbiol. 等相關(guān)國(guó)際期刊發(fā)表學(xué)術(shù)論文近30篇。與國(guó)際相關(guān)領(lǐng)域研究室保持密切合作關(guān)系，多次應(yīng)邀赴美國(guó)、德國(guó)、丹麥、英國(guó)、意大利、澳大利亞和日本等國(guó)家參加學(xué)術(shù)會(huì)議并進(jìn)行學(xué)術(shù)訪問(wèn)和交流。
　　
　　研究方向
　　主要開(kāi)展以下兩方面的研究：
　　1. 極端嗜熱古菌復(fù)制、重組及修復(fù)機(jī)制研究。以嗜熱古菌為材料，利用分子生物學(xué)、生物化學(xué)、遺傳學(xué)和結(jié)構(gòu)生物學(xué)等手段，研究重要蛋白質(zhì)的性質(zhì)、相互作用、結(jié)構(gòu)和體內(nèi)外功能，揭示古菌DNA重組修復(fù)的機(jī)理；
　　2. 資源環(huán)境微生物學(xué)研究。從極端環(huán)境如高溫、酸性和堿性或其它特殊環(huán)境中分離、鑒定微生物，鑒定生物質(zhì)降解相關(guān)的酶類；研究酶的性質(zhì)、結(jié)構(gòu)與功能，利用分子生物學(xué)方法改造酶，為極端環(huán)境微生物和極端酶的工業(yè)應(yīng)用奠定基礎(chǔ)。
　　
　　研究課題
　　1. 已主持或參與完成多項(xiàng)國(guó)家級(jí)研究項(xiàng)目，包括國(guó)家自然科學(xué)基金面上項(xiàng)目和國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃（973）項(xiàng)目。
　　2. 目前承擔(dān)國(guó)家自然科學(xué)基金重點(diǎn)項(xiàng)目一項(xiàng)及面上項(xiàng)目?jī)身?xiàng)。
　　
　　主講課程
　　極端環(huán)境微生物學(xué)及其專題講座
　　
　　招生情況
　　招生專業(yè): 生物化學(xué)與分子生物學(xué)、微生物學(xué)
　　每年招收碩士、博士研究生數(shù)名。另外接收博士后若干名。
　　
　　聯(lián)合培養(yǎng)情況及畢業(yè)生去向
　　與國(guó)內(nèi)外研究室保持著合作關(guān)系，優(yōu)秀學(xué)生可被選派到相關(guān)研究組聯(lián)合培養(yǎng)或深造。畢業(yè)生去向有：國(guó)內(nèi)大專院校教師、國(guó)內(nèi)生物技術(shù)公司職員及國(guó)外博士生或博士后等。
　　
　　發(fā)表論文：
　　1. Hong Y, Chu M, Li Y, Ni J, Sheng D, Hou G, She Q, Shen Y*. (2011) Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair, doi:10.1016/j.dnarep.2011. 10.009
　　2. Zheng T, Huang H, Zhang C, Ni J, She Q, Shen Y*. (2011) Development of simvastatin selection marker for hyperthermophilic acidophile, Appl. Environ. Microbiol., doi:10.1128/AEM.06095 -11
　　3. Wei T., Zhang S., Hou L., Ni, J., Sheng D., Hou G., Shen Y*. (2011) The carboxyl terminal of the archeal nuclease NurA is involved in the interaction with single-stranded DNA-binding protein and dimer formation. Extremophiles, 15：11-18.
　　4. Ma X., Hong Y., Han W., Sheng D. Ni, J. Hou G., Shen Y*. (2010) Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting, Extremophiles，14：1-10.
　　5. Wu L., Liu B., Hong Y., Sheng D., Shen Y*., Ni J. (2010) Residue Tyr224 is critical for the thermostability of Geobacillus sp. RD-2 lipase, Biotechnol Lett. 32:107-112.
　　6. Liu B., Wu L., Liu T., Hong Y., Shen Y*., Ni J. (2009) A MOFRL family glycerate kinase from the thermophilic crenarchaeon Sulfolobus tokodaii with unique enzymatic properties, Biotechnol Lett. 31:1937-1941.
　　7. Wang F, Zhang S. Huang Q, Shen Y* and Ni J. (2009) Advance in genetic manipulation systems of Hyperthermophilic archaea Acta Microbiologica Sinica 49, 1418-1423.
　　8. Huang Q, Shen Y and Ni, J. Optimization of selectivr conditions for the selection of uracil auxotrophs of thermophilic archaea Sulfolobus tokodaii. (2008) Journal of Shandong University (Natural Science), 43: 6-10.
　　9. Liu T, Shen Y, Liu Q, Liu B (2008) The unique Entner-Doudoroff (ED) glycolysis pathway of glucose in archaea--a review. Acta Microbiologica Sinica. 48:1126-31
　　10. Lu S, Li Z, Wang Z, Ma X, Sheng D, Ni J, Shen Y*. （2008）Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii. Biochem Biophys Res Commun. 376:369-74.
　　11. Li Z., Lu S., Hou G., Ma X., Sheng D., Ni J., and Shen Y*. (2008) Hjm/Hel308a DNA helicase from Sulfolobus tokodaii promotes replication fork regression and interacts with Hjc endonuclease in vitro. J. Bacteriol. 190: 3006–3017.
　　12. Zhang S., Wei W., Hou G., Zhang C., Liang P., Ni J., Sheng D., and Shen Y*. (2008) Archaeal DNA helicase HerA interacts with Mre11 homologue and unwinds blunt-ended double-stranded DNA and recombination intermediates. DNA Repair 7:380–391.
　　13. Wei T., Zhang S., Zhu S., Sheng D., Ni J., and Shen Y*. (2008) Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5'-3' nuclease NurA. Biochem. Biophys. Res. Commun. 367:523-9.
　　14. Sheng D., Li M., Jiao J., Ni J., and Shen Y*.（2008）Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaii. Sci. China C Life Sci. 51:60-5.
　　15. Sheng D., Zhu S., Wei T., Ni J., and Shen Y*. (2008) The in vitro activity of a Rad55 homologue from Sulfolobus tokodaii, a candidate mediator in RadA-catalyzed homologous recombination. Extremophiles 12:147-157.
　　16. Sheng D., Zhu S., Li M., Jiao, Ni J., and Shen Y*. (2008) Cloning, expression and radiation inducibility of RadA from the hyperthermophilic archaeon Sulfolobus tokodaii. Acta Microbiologica Sinica 48:1-6.
　　17. Hong Y., Wu L., Liu B., Peng C., Sheng D., Ni J, and Shen Y*. (2008) Characterization of a glucan phosphorylase from the thermophilic archaeon Sulfolobus tokodaii strain 7. J. Mol. Catal. B: Enzy. 54:27-34
　　18. Liu B., Hong Y., Wu L., Li Z., Ni J., Sheng D., and Shen Y*. (2007) A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic archaeon Pyrococcus horikoshii: gene cloning, expression and characterization. Extremophiles. 11:733-9.
　　19. Liu B., Li Z., Hong Y., Ni J., Sheng D., and Shen Y*. ( 2006) Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol. Lett. 28:1655-1660.
　　20. Liu B., Ni J., and Shen Y*. (2006) Cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilic archaeion Pyrococcus horikoshii. Acta Microbiologica Sinica 46:255-258.
　　21. Tang X.-F, Shen Y., Matsui E., Matsui I. (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii. Biochemistry. 43:11818-27.
　　22. Shen Y., Tang X.-F., Matsui E., and Matsui I. (2004) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerse from Pyrococcus horikoshii. Biochem. Soc. Trans. 32:245-9.
　　23. Shen Y., Tang X.-F., Yokoyama H., Matsui E., and Matsui I. (2004) A 21-aa peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the co-factor. Nucl. Acids Res. 32: 158-68.
　　24. Shen Y., Tang X.-F., and Matsui I. (2003) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. J. Biol. Chem. 278:21247-57.
　　25. Shen Y., Musti K., Hiramoto M., Kikuchi H., Kawabayashi Y., and Matsui, I. (2001) Invariant Asp1122 and Asp1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii. J. Biol. Chem. 276: 27376-83.
　　26. Zheng R., Matsui E., Shen Y., Kishnasastry M., Feng Y., Darnis S., Kawarabayasi Y., Kikuchi H., Harata K., and Matsui I. (2001) The novel function of a short region K 253 XRXXXD 259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii. Extremophiles 5:111-7.
　　27. Shen Y., Newbury H. J., and Ford-Lloyd B. V. (1998) Identification of taxa in the genus Beta using ITS1 sequence information. Plant Mol. Biol. Rep. 16: 147-55.
　　28. Shen Y., Ford-Lloyd B. V., and Newbury H. J. (1998) Genetic relationship using both PCR-based and DNA sequencing methods. Heredity 80: 624-32.
　　29. Shen Y., Newbury H. J., and Ford-Lloyd B. V. (1996) The taxonomic characterization of annual Beta germplasm in a genetic resources collection using RAPD makers. Euphytica 91: 205-12.

　　*如果發(fā)現(xiàn)導(dǎo)師信息存在錯(cuò)誤或者偏差，歡迎隨時(shí)與我們聯(lián)系，以便進(jìn)行更新完善。

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